Serum-free Hybridoma Growth Medium
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Serum-free Hybridoma Growth Medium

  • It supports the cultivation of hybridoma multi-cell lines and can be used for the cultivation of mouse, rat, and hamster hybridoma cells. In most cases, direct transfer is possible without the need for acclimation. 

  • The antibody expression level is stable. The antibody concentration in static culture is greater than 300 mg/L, and the antibody concentration in dynamic culture is greater than 500 mg/L. At the same time, the average cell doubling time is one day faster than that of competing products. 

  • It is suitable for large-scale production and applicable to various systems such as shake flasks, culture bags, and bioreactors, meeting different production and research-and-development scales. 

Availability:

The Serum-free Hybridoma Growth Medium is designed for optimal cultivation of hybridoma cell lines, including mouse, rat, and hamster hybridomas, without the need for animal serum. This medium offers enhanced stability in antibody expression, with antibody concentrations reaching over 300 mg/L in static culture and 500 mg/L in dynamic culture. It accelerates cell growth, achieving a faster doubling time compared to other products. Suitable for large-scale production, it supports various systems such as shake flasks, culture bags, and bioreactors, making it ideal for both research and clinical applications. With minimal batch-to-batch variation and compliance with GMP standards, it ensures reliable and high-quality results for monoclonal antibody production.


Specifications and Packaging 

  • Product Name: Serum-free Hybridoma Growth Medium

  • Item No.: NC0301

  • Volume: 1000 mL per bottle

  • Cell Line Compatibility: Suitable for mouse, rat, and hamster hybridoma cells

  • Antibody Production: Over 300 mg/L in static culture and 500 mg/L in dynamic culture

  • Cultivation Systems: Compatible with shake flasks, culture bags, and bioreactors

  • Standards: Manufactured according to GMP standards for consistency and quality

  • Batch Consistency: Offers minimal batch-to-batch variation for reliable results across production cycles


Product Advantage

High Antibody Production

The Serum-free Hybridoma Growth Medium ensures superior antibody yields, with concentrations reaching 300 mg/L in static culture and 500 mg/L in dynamic culture, making it an excellent choice for large-scale monoclonal antibody production.

Faster Cell Doubling Time

This medium promotes a faster cell doubling time, allowing for quicker cultivation and improving overall production efficiency compared to competing products.

Scalability for Large-Scale Production

The medium is suitable for a variety of cultivation systems, including shake flasks, culture bags, and bioreactors, ensuring seamless scalability from research to large-scale manufacturing.

Minimal Batch-to-Batch Variation

Designed with consistency in mind, the medium offers minimal batch-to-batch variation, ensuring stable performance across different production cycles.

GMP Compliance

Manufactured under GMP standards, the Serum-free Hybridoma Growth Medium is ideal for clinical research and ensures high-quality, reliable results for sensitive applications.


Serum-free Hybridoma Growth Medium



Product Application

Monoclonal Antibody Production

The Serum-free Hybridoma Growth Medium is specifically designed for the efficient cultivation of hybridoma cells, making it an ideal choice for monoclonal antibody production. Its high antibody yield and stable expression support various antibody-based applications in research and clinical settings.

Large-Scale Cell Culture

Suitable for large-scale production, the medium supports various systems such as shake flasks, culture bags, and bioreactors. This flexibility enables seamless transition from small-scale research to large-scale manufacturing, ensuring efficiency and scalability.

Clinical Research Applications

With minimal batch-to-batch variation and GMP compliance, the Serum-free Hybridoma Growth Medium is perfectly suited for clinical research, providing reliable and consistent results in sensitive medical and therapeutic research environments.

Cell Line Development

The medium supports the growth of mouse, rat, and hamster hybridoma cells, making it ideal for creating and maintaining robust hybridoma cell lines used in various diagnostic and therapeutic applications.


The Serum-free Hybridoma Growth Medium is an advanced solution designed to optimize hybridoma cell cultivation, including mouse, rat, and hamster hybridomas, without the need for animal serum. Offering high antibody yields, faster cell doubling time, and scalability for large-scale production, this medium ensures consistent performance and minimal batch-to-batch variation. Compliant with GMP standards, it is ideal for both research and clinical applications, particularly in monoclonal antibody production.

Unlock reliable, high-quality results with the Serum-free Hybridoma Growth Medium, and take your antibody production to the next level.


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