The High-Performance NK Cell Expansion Medium is a serum-free solution designed for optimal expansion and activation of NK cells from challenging samples like cord blood or peripheral blood with extended transport or uncertain cryopreservation quality. It offers up to 200-fold expansion and over 90% positivity after 14-16 days, without the need for autologous plasma or feeder cells. This medium is compatible with both research-grade and chemically defined basal media, ensuring simple and consistent culture methods. FDA DMF filed, it guarantees high-quality results and is ideal for large-scale NK cell culture, scaling from 2L to 4L systems.
Unlock the full potential of NK cell culture with this advanced, scalable solution.
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Overview
The high-performance version can obtain more than 200 times of cell expansion and more than 90% of positive rate after 14~16 days of culturing. It can be specifically used for the activation and culture of NK cells in unconventional cord blood or peripheral blood samples, such as blood samples with a long-distance transportation time of more than 8 hours or unknown cryopreservation quality, and supports the culture protocol without the addition of autologous plasma.
Induction with pure factors, with no trophoblastic cells, simple and feasible culture method, basically uniform cell state, fed-batch of solutions according to the recommended process, without the need for cell counting. When combined with the newly upgraded NK5.0 basic culture medium, it significantly improves the continuous expansion ability of cells after 7 consecutive days of cultivation, and the continuous support ability of cells that expand from 2 L system to 3 L or even 4 L system after 14 days.
It can be paired with the research-grade basal medium (NC0102) or the chemically-defined basal medium (NC0102.H). The basal medium has been filed with the US FDA DMF with the filing No. of 37874.
Overview
The high-performance version can obtain more than 200 times of cell expansion and more than 90% of positive rate after 14~16 days of culturing. It can be specifically used for the activation and culture of NK cells in unconventional cord blood or peripheral blood samples, such as blood samples with a long-distance transportation time of more than 8 hours or unknown cryopreservation quality, and supports the culture protocol without the addition of autologous plasma.
Induction with pure factors, with no trophoblastic cells, simple and feasible culture method, basically uniform cell state, fed-batch of solutions according to the recommended process, without the need for cell counting. When combined with the newly upgraded NK5.0 basic culture medium, it significantly improves the continuous expansion ability of cells after 7 consecutive days of cultivation, and the continuous support ability of cells that expand from 2 L system to 3 L or even 4 L system after 14 days.
It can be paired with the research-grade basal medium (NC0102) or the chemically-defined basal medium (NC0102.H). The basal medium has been filed with the US FDA DMF with the filing No. of 37874.
Version | Application Scope | Product item number | |
Inoculation blood volume | Item No. | ||
Standard Version | 1. Sample initial positivity rate above 10%; 2. No specific requirement for extremely high positivity rate (above 90%); 3. Can accept special samples with positivity rate below 70%. | 30mL | NC0102+AN0107 |
50mL | NC0102+AN0107-2 | ||
High-Performance Version | 1. Samples with low initial positivity rate or not tested; 2. High demand for extremely high positivity rate (above 90%); 3. Pursuit of extremely high success rate and fault tolerance. As well as the following special circumstances: 1. Samples transported for a long time (transport time exceeding 8 hours); 2. Frozen samples that used non-YOCON mononuclear cell cryopreservation solution; 3. No autologous plasma available for addition. | 30mL | NC0102.F +NC0102+AN0104 |
50mL | NC0102.F +NC0102+AN0104-2 |
*All the above-mentioned basal media can be combined with the research-grade serum-free NK cell culture medium (NC0102) or the chemically defined serum-free NK cell culture medium (NC0102.H)
Expansion Multiple:
High-Performance Version: Achieved an average of 202-fold expansion after 14-16 days.
Standard Version: Achieved 184-fold expansion after 14-16 days.
Difference: The high-performance version provides a higher expansion rate, but the fluctuation range for both versions is similar.
Positivity Rate:
High-Performance Version: Achieved an average positivity rate of 92.74%, with minimal fluctuation, unaffected by the initial sample level.
Standard Version: Achieved an average positivity rate of 71.22%, with a larger fluctuation range.
Data Consistency:
The High-Performance Version shows more stable and consistent results across different sample types (hemolytic, fresh, cryopreserved), while the Standard Version exhibits higher variability in both expansion and positivity rates.
Version | Application Scope | Product item number | |
Inoculation blood volume | Item No. | ||
Standard Version | 1. Sample initial positivity rate above 10%; 2. No specific requirement for extremely high positivity rate (above 90%); 3. Can accept special samples with positivity rate below 70%. | 30mL | NC0102+AN0107 |
50mL | NC0102+AN0107-2 | ||
High-Performance Version | 1. Samples with low initial positivity rate or not tested; 2. High demand for extremely high positivity rate (above 90%); 3. Pursuit of extremely high success rate and fault tolerance. As well as the following special circumstances: 1. Samples transported for a long time (transport time exceeding 8 hours); 2. Frozen samples that used non-YOCON mononuclear cell cryopreservation solution; 3. No autologous plasma available for addition. | 30mL | NC0102.F +NC0102+AN0104 |
50mL | NC0102.F +NC0102+AN0104-2 |
*All the above-mentioned basal media can be combined with the research-grade serum-free NK cell culture medium (NC0102) or the chemically defined serum-free NK cell culture medium (NC0102.H)
Expansion Multiple:
High-Performance Version: Achieved an average of 202-fold expansion after 14-16 days.
Standard Version: Achieved 184-fold expansion after 14-16 days.
Difference: The high-performance version provides a higher expansion rate, but the fluctuation range for both versions is similar.
Positivity Rate:
High-Performance Version: Achieved an average positivity rate of 92.74%, with minimal fluctuation, unaffected by the initial sample level.
Standard Version: Achieved an average positivity rate of 71.22%, with a larger fluctuation range.
Data Consistency:
The High-Performance Version shows more stable and consistent results across different sample types (hemolytic, fresh, cryopreserved), while the Standard Version exhibits higher variability in both expansion and positivity rates.
The High-Performance NK Cell Expansion Medium enables up to 200-fold cell expansion and achieves a positivity rate over 85% after 14-16 days of culture, making it highly efficient for NK cell culture.
This medium supports NK cell culture without the need for autologous plasma, simplifying the process and making it suitable for unconventional blood samples, including those with unknown cryopreservation quality.
The culture method is easy to perform with no need for cell counting. Cells are cultured with a straightforward, feed-batch system, ensuring uniformity and consistency in cell state throughout the process.
Compatible with both research-grade basal media and chemically-defined basal media, the medium provides flexibility to suit different experimental setups and requirements.
This medium supports the continuous expansion ability of NK cells when scaling from 2L to 4L systems, making it ideal for both small and large-scale NK cell culture applications.
The High-Performance NK Cell Expansion Medium is FDA DMF filed (FDA filing No. 37874), ensuring compliance with regulatory standards and consistent, high-quality results for research and clinical applications.
The High-Performance NK Cell Expansion Medium enables up to 200-fold cell expansion and achieves a positivity rate over 85% after 14-16 days of culture, making it highly efficient for NK cell culture.
This medium supports NK cell culture without the need for autologous plasma, simplifying the process and making it suitable for unconventional blood samples, including those with unknown cryopreservation quality.
The culture method is easy to perform with no need for cell counting. Cells are cultured with a straightforward, feed-batch system, ensuring uniformity and consistency in cell state throughout the process.
Compatible with both research-grade basal media and chemically-defined basal media, the medium provides flexibility to suit different experimental setups and requirements.
This medium supports the continuous expansion ability of NK cells when scaling from 2L to 4L systems, making it ideal for both small and large-scale NK cell culture applications.
The High-Performance NK Cell Expansion Medium is FDA DMF filed (FDA filing No. 37874), ensuring compliance with regulatory standards and consistent, high-quality results for research and clinical applications.
The High-Performance NK Cell Expansion Medium is highly effective in culturing NK cells from cord blood samples, including those that have undergone long-distance transportation (more than 8 hours). It ensures robust cell expansion and high positivity rates, making it ideal for unconventional samples.
This medium is perfect for expanding NK cells from peripheral blood samples, including those from elderly individuals or other challenging conditions. It has shown to be highly successful even in samples transported for extended periods, achieving high positivity rates and significant cell expansion.
The High-Performance NK Cell Expansion Medium supports large-scale culture applications, from 2L systems to 3L or 4L systems. This makes it a go-to solution for researchers needing consistent and scalable expansion of NK cells for therapeutic or research purposes.
A cord blood sample was transported for 23 hours and cultured using the high-performance version of the medium. After 21 days, the cells expanded to 18.9 billion cells with a positive rate of 88.26% in a 4.5L system, demonstrating the medium's ability to handle challenging conditions.
A 62-year-old patient’s peripheral blood sample was cultured using the medium after being transported for 18 hours. After 16 days, the cells reached 8.7 billion cells with a positive rate of 98.72% in a 3L system, illustrating the medium’s effectiveness even in aged samples.
The High-Performance NK Cell Expansion Medium is highly effective in culturing NK cells from cord blood samples, including those that have undergone long-distance transportation (more than 8 hours). It ensures robust cell expansion and high positivity rates, making it ideal for unconventional samples.
This medium is perfect for expanding NK cells from peripheral blood samples, including those from elderly individuals or other challenging conditions. It has shown to be highly successful even in samples transported for extended periods, achieving high positivity rates and significant cell expansion.
The High-Performance NK Cell Expansion Medium supports large-scale culture applications, from 2L systems to 3L or 4L systems. This makes it a go-to solution for researchers needing consistent and scalable expansion of NK cells for therapeutic or research purposes.
A cord blood sample was transported for 23 hours and cultured using the high-performance version of the medium. After 21 days, the cells expanded to 18.9 billion cells with a positive rate of 88.26% in a 4.5L system, demonstrating the medium's ability to handle challenging conditions.
A 62-year-old patient’s peripheral blood sample was cultured using the medium after being transported for 18 hours. After 16 days, the cells reached 8.7 billion cells with a positive rate of 98.72% in a 3L system, illustrating the medium’s effectiveness even in aged samples.
Technical Specifications
The High-Performance NK Cell Expansion Medium provides up to 200-fold cell expansion after 14-16 days of culture, ensuring significant proliferation of NK cells from various sample types.
The medium achieves a positivity rate of over 90%, making it ideal for high-efficiency NK cell culture, even from unconventional or difficult samples.
This medium is compatible with both research-grade basal media (NC0102) and chemically-defined basal media (NC0102.H), offering flexibility in experimental setups.
The High-Performance NK Cell Expansion Medium is FDA DMF filed (filing No. 37874), ensuring compliance with regulatory standards for quality and consistency.
The culture method is simple and effective, requiring no cell counting and using a feed-batch system to maintain consistent cell growth throughout the process.
This medium supports scalable NK cell expansion, from 2L systems to larger 3L or 4L systems, enabling both small and large-scale culture applications.
Technical Specifications
The High-Performance NK Cell Expansion Medium provides up to 200-fold cell expansion after 14-16 days of culture, ensuring significant proliferation of NK cells from various sample types.
The medium achieves a positivity rate of over 90%, making it ideal for high-efficiency NK cell culture, even from unconventional or difficult samples.
This medium is compatible with both research-grade basal media (NC0102) and chemically-defined basal media (NC0102.H), offering flexibility in experimental setups.
The High-Performance NK Cell Expansion Medium is FDA DMF filed (filing No. 37874), ensuring compliance with regulatory standards for quality and consistency.
The culture method is simple and effective, requiring no cell counting and using a feed-batch system to maintain consistent cell growth throughout the process.
This medium supports scalable NK cell expansion, from 2L systems to larger 3L or 4L systems, enabling both small and large-scale culture applications.