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The GMP Grade Serum-Free MSC Culture Medium is specifically designed for the primary isolation, seed bank establishment, and continuous subculture of human mesenchymal stem cells (MSCs) from sources like bone marrow and umbilical cord. This medium supports MSCs’ multipotential differentiation capacity without the need for serum or substitutes, ensuring a defined, animal- and human-free composition. It offers minimal batch-to-batch variation and complies with GMP standards, making it ideal for clinical research and regenerative medicine. The medium maintains cell morphology and phenotype while preserving the undifferentiated state of stem cells.
Product Name: Mesenchymal Stem Cells Serum-free Medium (GMP Grade) 2.0
Cat. No.: NC0107
Packaging Specification: 500 mL/bottle
Storage Conditions: 2-8°C, protect from light
Shelf Life: 12 months
Appearance: Light yellow to yellow, clear, and transparent liquid
Fill Volume: 500 mL/bottle
Osmolarity: 280-350 mOsm/Kg
Endotoxin: <0.25 EU/mL
Sterility: No bacteria or fungi detected
Mycoplasma: Not detected
pH Value: 6.8-7.8 at 25°C
Visible Cell Growth: 5-9 days (Primary isolation from human umbilical cord)
Cell Harvest Time: 12-16 days
Time for Cell Passage: 3-4 days
Seeding Density: 8000-15000 cells/cm²
Negative for: CD14, CD19, CD34, CD45, and HLA-DR (<2%)
Positive for: CD44, CD73, CD90, and CD105 (>95%)
Shape: Spindle-shaped, growing in a fingerprint or whirlpool pattern
The GMP Grade Serum-Free MSC Culture Medium eliminates the need for serum or serum substitutes, ensuring a defined chemical composition that supports mesenchymal stem cells (MSCs) without the risk of differentiation.
All raw materials comply with GMP standards, making it ideal for clinical research applications, ensuring consistency and high-quality results.
The medium is formulated to have minimal batch-to-batch variation, ensuring reliable and reproducible outcomes for MSC culture.
This medium maintains the multipotential differentiation capacity of MSCs, which is essential for their use in regenerative medicine and stem cell therapies.
The medium supports normal cell morphology and phenotype, maintaining the undifferentiated state of MSCs during culture, essential for their therapeutic potential.
Effective for primary isolation, seed bank establishment, and continuous subculture of MSCs from various sources like bone marrow and umbilical cord.
The GMP Grade Serum-Free MSC Culture Medium is ideal for primary isolation of mesenchymal stem cells (MSCs) from human umbilical cord and bone marrow. It ensures a serum-free environment that preserves the undifferentiated state of the cells during isolation.
This medium is highly effective for seed bank establishment, supporting long-term storage and reliable subculture of MSCs, making it a valuable tool for stem cell banking and preservation.
The medium is designed to support the continuous subculture of MSCs, allowing for the maintenance of normal cell morphology and multipotential differentiation capacity without the need for serum or serum substitutes.
Ideal for clinical research applications, this serum-free medium meets GMP standards, ensuring high-quality, reproducible results that are critical for regenerative medicine and stem cell therapies.
The product supports regenerative medicine by maintaining the multipotential differentiation capacity of MSCs, which is essential for their therapeutic use in tissue repair and regeneration.
Product Name: Mesenchymal Stem Cells Serum-free Medium (GMP Grade) 2.0
Storage Conditions:
Medium: Store at 2-8°C, protect from light.
Supplement: Store at -20°C, protect from light.
Shelf Life: 12 months
Storage Recommendations:
Keep the product tightly sealed and stored in the original packaging to preserve its stability and effectiveness.
Avoid exposure to direct light to maintain product integrity.
Primary Cell Isolation from Human Umbilical Cord:
Visible Cell Growth: 5-9 days
Cell Harvest Time: 12-16 days
Note: For edematous or cryopreserved umbilical cord tissue, visible MSC outgrowth may take about 14 days, and cell harvest may occur after 21 days.
Time Required for Cell Passage: 3-4 days
P1-P5: 8000 cells/cm²
P6-P10: 10000 cells/cm²
P11-P15: 12000 cells/cm²
P16-P20: 15000 cells/cm²
Seeding Density: 8000-15000 cells/cm²
Passage density progression:
Cell Phenotype:
Negative for: CD14, CD19, CD34, CD45, and HLA-DR (<2%)
Positive for: CD44, CD73, CD90, and CD105 (>95%)
Cell Morphology:
Spindle-shaped, growing in a fingerprint or whirlpool pattern.
Product Specifications:
Osmolarity: 280-350 mOsm/Kg
Endotoxin: <0.25 EU/mL
Sterility: No bacteria or fungi detected
Mycoplasma: Not detected
pH Value: 6.8-7.8 at 25°C
Appearance: Light yellow to yellow, clear, and transparent liquid
Fill Volume: 500 mL/bottle
The GMP Grade Serum-Free MSC Culture Medium is a serum-free solution designed for the isolation, seed bank establishment, and continuous subculture of human mesenchymal stem cells (MSCs) from sources like bone marrow and umbilical cord. With a defined chemical composition and GMP standards compliance, it maintains the multipotential differentiation capacity of MSCs while preserving their undifferentiated state. This medium offers minimal batch-to-batch variation, ensuring reliable results for clinical research and regenerative medicine.
Ensure high-quality MSC culture for your research—order now!
The GMP Grade Serum-Free MSC Culture Medium is specifically designed for the primary isolation, seed bank establishment, and continuous subculture of human mesenchymal stem cells (MSCs) from sources like bone marrow and umbilical cord. This medium supports MSCs’ multipotential differentiation capacity without the need for serum or substitutes, ensuring a defined, animal- and human-free composition. It offers minimal batch-to-batch variation and complies with GMP standards, making it ideal for clinical research and regenerative medicine. The medium maintains cell morphology and phenotype while preserving the undifferentiated state of stem cells.
Product Name: Mesenchymal Stem Cells Serum-free Medium (GMP Grade) 2.0
Cat. No.: NC0107
Packaging Specification: 500 mL/bottle
Storage Conditions: 2-8°C, protect from light
Shelf Life: 12 months
Appearance: Light yellow to yellow, clear, and transparent liquid
Fill Volume: 500 mL/bottle
Osmolarity: 280-350 mOsm/Kg
Endotoxin: <0.25 EU/mL
Sterility: No bacteria or fungi detected
Mycoplasma: Not detected
pH Value: 6.8-7.8 at 25°C
Visible Cell Growth: 5-9 days (Primary isolation from human umbilical cord)
Cell Harvest Time: 12-16 days
Time for Cell Passage: 3-4 days
Seeding Density: 8000-15000 cells/cm²
Negative for: CD14, CD19, CD34, CD45, and HLA-DR (<2%)
Positive for: CD44, CD73, CD90, and CD105 (>95%)
Shape: Spindle-shaped, growing in a fingerprint or whirlpool pattern
The GMP Grade Serum-Free MSC Culture Medium eliminates the need for serum or serum substitutes, ensuring a defined chemical composition that supports mesenchymal stem cells (MSCs) without the risk of differentiation.
All raw materials comply with GMP standards, making it ideal for clinical research applications, ensuring consistency and high-quality results.
The medium is formulated to have minimal batch-to-batch variation, ensuring reliable and reproducible outcomes for MSC culture.
This medium maintains the multipotential differentiation capacity of MSCs, which is essential for their use in regenerative medicine and stem cell therapies.
The medium supports normal cell morphology and phenotype, maintaining the undifferentiated state of MSCs during culture, essential for their therapeutic potential.
Effective for primary isolation, seed bank establishment, and continuous subculture of MSCs from various sources like bone marrow and umbilical cord.
The GMP Grade Serum-Free MSC Culture Medium is ideal for primary isolation of mesenchymal stem cells (MSCs) from human umbilical cord and bone marrow. It ensures a serum-free environment that preserves the undifferentiated state of the cells during isolation.
This medium is highly effective for seed bank establishment, supporting long-term storage and reliable subculture of MSCs, making it a valuable tool for stem cell banking and preservation.
The medium is designed to support the continuous subculture of MSCs, allowing for the maintenance of normal cell morphology and multipotential differentiation capacity without the need for serum or serum substitutes.
Ideal for clinical research applications, this serum-free medium meets GMP standards, ensuring high-quality, reproducible results that are critical for regenerative medicine and stem cell therapies.
The product supports regenerative medicine by maintaining the multipotential differentiation capacity of MSCs, which is essential for their therapeutic use in tissue repair and regeneration.
Product Name: Mesenchymal Stem Cells Serum-free Medium (GMP Grade) 2.0
Storage Conditions:
Medium: Store at 2-8°C, protect from light.
Supplement: Store at -20°C, protect from light.
Shelf Life: 12 months
Storage Recommendations:
Keep the product tightly sealed and stored in the original packaging to preserve its stability and effectiveness.
Avoid exposure to direct light to maintain product integrity.
Primary Cell Isolation from Human Umbilical Cord:
Visible Cell Growth: 5-9 days
Cell Harvest Time: 12-16 days
Note: For edematous or cryopreserved umbilical cord tissue, visible MSC outgrowth may take about 14 days, and cell harvest may occur after 21 days.
Time Required for Cell Passage: 3-4 days
P1-P5: 8000 cells/cm²
P6-P10: 10000 cells/cm²
P11-P15: 12000 cells/cm²
P16-P20: 15000 cells/cm²
Seeding Density: 8000-15000 cells/cm²
Passage density progression:
Cell Phenotype:
Negative for: CD14, CD19, CD34, CD45, and HLA-DR (<2%)
Positive for: CD44, CD73, CD90, and CD105 (>95%)
Cell Morphology:
Spindle-shaped, growing in a fingerprint or whirlpool pattern.
Product Specifications:
Osmolarity: 280-350 mOsm/Kg
Endotoxin: <0.25 EU/mL
Sterility: No bacteria or fungi detected
Mycoplasma: Not detected
pH Value: 6.8-7.8 at 25°C
Appearance: Light yellow to yellow, clear, and transparent liquid
Fill Volume: 500 mL/bottle
The GMP Grade Serum-Free MSC Culture Medium is a serum-free solution designed for the isolation, seed bank establishment, and continuous subculture of human mesenchymal stem cells (MSCs) from sources like bone marrow and umbilical cord. With a defined chemical composition and GMP standards compliance, it maintains the multipotential differentiation capacity of MSCs while preserving their undifferentiated state. This medium offers minimal batch-to-batch variation, ensuring reliable results for clinical research and regenerative medicine.
Ensure high-quality MSC culture for your research—order now!
