GMP Grade Serum-Free MSC Culture Medium

Product Specifications

• Product Name: Mesenchymal Stem Cells Serum-free Medium (GMP Grade) 2.0

• Cat. No.: NC0107

• Packaging Specification: 500 mL/bottle

• Storage Conditions: 2-8°C, protect from light

• Shelf Life: 12 months

• Appearance: Light yellow to yellow, clear, and transparent liquid

•  Fill Volume: 500 mL/bottle

Key Performance Indicators:

• Osmolarity: 280-350 mOsm/Kg

• Endotoxin: <0.25 EU/mL

• Sterility: No bacteria or fungi detected

• Mycoplasma: Not detected

• pH Value: 6.8-7.8 at 25°C

Cell Culture Data:

• Visible Cell Growth: 5-9 days (Primary isolation from human umbilical cord)

• Cell Harvest Time: 12-16 days

• Time for Cell Passage: 3-4 days

  Note: For edematous or cryopreserved umbilical cord tissue, visible MSC outgrowth may take about 14 days, and cell harvest may occur after 21 days.

• Seeding Density: 8000-15000 cells/cm²

Cell Phenotype:

• Negative for: CD14, CD19, CD34, CD45, and HLA-DR (<2%)

• Positive for: CD44, CD73, CD90, and CD105 (>95%)

Cell Morphology:

• Shape: Spindle-shaped, growing in a fingerprint or whirlpool pattern

Product Advantage

• Serum-Free and Defined Composition

The GMP Grade Serum-Free MSC Culture Medium eliminates the need for serum or serum substitutes, ensuring a defined chemical composition that supports mesenchymal stem cells (MSCs) without the risk of differentiation.

• Compliant with GMP Standards

All raw materials comply with GMP standards, making it ideal for clinical research applications, ensuring consistency and high-quality results.

• Minimal Batch-to-Batch Variation

The medium is formulated to have minimal batch-to-batch variation, ensuring reliable and reproducible outcomes for MSC culture.

• Supports Multipotential Differentiation

This medium maintains the multipotential differentiation capacity of MSCs, which is essential for their use in regenerative medicine and stem cell therapies.

• Optimized for Cell Morphology and Phenotype

The medium supports normal cell morphology and phenotype, maintaining the undifferentiated state of MSCs during culture, essential for their therapeutic potential.

• Wide Compatibility with MSC Sources

Effective for primary isolation, seed bank establishment, and continuous subculture of MSCs from various sources like bone marrow and umbilical cord.

Product Application

• Primary MSC Isolation

The GMP Grade Serum-Free MSC Culture Medium is ideal for primary isolation of mesenchymal stem cells (MSCs) from human umbilical cord and bone marrow. It ensures a serum-free environment that preserves the undifferentiated state of the cells during isolation.

• Seed Bank Establishment

This medium is highly effective for seed bank establishment, supporting long-term storage and reliable subculture of MSCs, making it a valuable tool for stem cell banking and preservation.

• Continuous Subculture of MSCs

The medium is designed to support the continuous subculture of MSCs, allowing for the maintenance of normal cell morphology and multipotential differentiation capacity without the need for serum or serum substitutes.

• Clinical Research Applications

Ideal for clinical research applications, this serum-free medium meets GMP standards, ensuring high-quality, reproducible results that are critical for regenerative medicine and stem cell therapies.

• Regenerative Medicine and Stem Cell Therapies

The product supports regenerative medicine by maintaining the multipotential differentiation capacity of MSCs, which is essential for their therapeutic use in tissue repair and regeneration.

Partial performance demonstration

Data of continuous subculturing

Notes :The data listed in the table are the average values from multiple tests. The actual performance may fluctuate slightlyabove or below these values, but the difference wil not be significant, For normal cell lines, the expansion multiplesfrom P1 to p5 are all above 9-fold, and those from P6 to P10 are all above 8 - fold. in addition, there is a margin of errorin cell counting. An expansion multiple difference of 0.5-1 fold is considered normal.

Exosome culture Data

NC0107 medium is used for exosome production in cell factories.

Thaw p2 cells and continuously passage them to P3 cells, then seed them into the cell factory, A total of 2.52x1012exosomes are harvested in 15 days.

Calculation of the Total Amount of Harvested Exosomes

Harvesting Exosomes from Cells Cultured in 3D on Microcarriers Using Nc0107 Medium.

Thaw P2 cells, seed p3 cells onto Yocon 3D microcarriers (0.5 g), With a culture cycle of 15 davs and 500 ml of the medium, atotal of 3.82x1012 exosomes are harvested.

Calculation of the Total Amount of Harvested Exosomes

Cell morphology

Identification of cell surface markers-P10