CIK Cell Serum-Free Expansion Medium
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CIK Cell Serum-Free Expansion Medium

  • Designed to support the robust growth and expansion of Cytokine-Induced Killer (CIK) cells, achieving remarkable proliferation rates essential for clinical and research applications.
  • Achieve remarkable cell expansion with over 200 times growth after just 14 days of culture in a 2L system, ensuring abundant cell yield for your research or therapeutic needs.
  • Simplify your culturing process through pure factor induction, eliminating the complexities associated with trophoblast cells and enabling a more efficient and straightforward cell expansion protocol.
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YOCONCELL CIK Cell Serum-Free Medium is a specialized culture solution meticulously developed for the efficient and large-scale ex vivo expansion of CIK cells. This innovative medium promotes superior cell proliferation and maintains the desired CIK phenotype (CD3+CD56+) without the complexities associated with serum-containing media or feeder cells. When used in conjunction with our newly upgraded basic immune cell culture medium, it further enhances the sustained growth of CIK cells, allowing for seamless scalability from 2L to 4L culture systems.


Product Information

Sample Type Inoculated  Blood Volume Version  Selection Item No. Performance Summary
Peripheral blood CIK 30mL Standard  Version NC0101 +AN0108 The total number of cells  harvested in a 2L system  is greater than 6 billion,  and the CIK phenotype is  greater than 15%.
High-Performance  Version NC0102.F +NC0101+AN0108
Umbilical cord  blood CIK 50mL Standard  Version NC0101 +AN0109-2 The total number of cells  harvested in a 2L system  is greater than 6 billion,  and the CIK phenotype is  greater than 20%.
High-Performance  Version NC0102.F +NC0101+AN0109-2


Features

Exceptional Cell Proliferation

Experience a remarkable increase in cell numbers, with over 200-fold expansion in a 2L culture system after 14 days, ensuring a robust supply of CIK cells.

Stable Phenotype Maintenance

Our medium guarantees the stability of the crucial CD3+CD56+ phenotype, maintaining it above 20% in a 2L culture system after 14 days, which is vital for the functional integrity of CIK cells.

Simplified & Efficient Induction

Benefit from a streamlined cell culture process thanks to our pure factor induction, which eliminates the need for complex trophoblast cell co-culture.

Enhanced Scalability & Performance

When used with our advanced basic immune cell culture medium, this product significantly boosts continuous cell expansion, supporting seamless transitions from 2L to 3L or even 4L systems after 14 days.

Regulatory Assurance

The basic culture medium is filed with the FDA DMF (Filing No. 37874), providing an extra layer of confidence in its quality and compliance.


Performance Display

Comparison of ClK culture Effects between Peripheral Blood and Umbilical Cord Blood

After 14 - 16 days of culture, the  average total number of peripheral  blood CIK cells is above 7.5 billion,  and the average positive rate is 44%.  The average total number of umbilical  cord blood CIK cells is above 9.58  billion, and the average positive rate  is 28%.


Advantage

Superior Cell Yields: Experience higher returns with an average of over 7.5 billion peripheral blood CIK cells (44% positive rate) and over 9.58 billion umbilical cord blood CIK cells (28% positive rate) after 14-16 days of culture.

Streamlining processes: Benefit from a simplified and more manageable cell culture process due to pure factor induction and the absence of trophoblast cells.

Scalability for Diverse Needs: Seamlessly scale your operations from 2L up to 4L systems, providing flexibility for varying research demands and potential clinical applications.

Assured Quality & Safety: The FDA DMF filing of the basic culture medium offers an added layer of trust and reliability, adhering to stringent regulatory standards.


Application

This specialized serum-free medium is designed for the efficient induction and robust expansion of Cytokine-Induced Killer (CIK) cells, making it an indispensable tool for:

  • Peripheral Blood CIK Cell Culture: Optimally supports the growth and expansion of CIK cells derived from peripheral blood, suitable for various research and clinical applications.

  • Umbilical Cord Blood CIK Cell Culture: Provides superior performance for the expansion of CIK cells sourced from umbilical cord blood, facilitating promising new avenues in cell therapy and research.

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