Adipose-Derived Mesenchymal Stem Cell Serum-Free Medium
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Adipose-Derived Mesenchymal Stem Cell Serum-Free Medium

  • This serum-free medium is specifically formulated for the primary isolation and continuous subculture of adipose-derived mesenchymal stem cells (AD-MSCs), offering a dedicated solution for your cell culture needs.
  • Achieve outstanding cell yields, with the capability to produce 4.5x10⁶ primary cells from just 10 mL of adipose tissue, and maintain robust cell growth through continuous subculture up to P20 without the need for medium changes.
  • Embrace a pure factor system that is entirely free of human-derived or animal-derived components, ensuring superior safety, consistency, and compliance with GMP standards crucial for clinical research applications.
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YOCON BIOTECH COMPANY's Adipose-Derived Mesenchymal Stem Cell Serum-Free Medium is a meticulously developed and high-performance culture solution designed for the robust proliferation and maintenance of human mesenchymal stem cells (hMSCs) from diverse sources, including adipose tissue, bone marrow (BM-hMSC), and umbilical cord (UCM-hMSC). This innovative medium eliminates the necessity for serum or serum substitutes, providing a precisely defined, animal-component-free formulation that significantly minimizes batch-to-batch variation.


Product Information

Product name    Item No. Specification Purpose
Base of serum-free medium for mesenchymal  stem cells NC0103 500 mL/bottle  5 mL of NC0104.S added to 500 mL of NC0103 basic  medium can be used for primary isolation and  subculture of adipose-derived mesenchymal stem  cells
Base of serum-free medium for mesenchymal  stem cells NC0104.S 5 mL/bottle
Adipose tissue digestive enzymes NC1005 100 mL/bottle Specially used for the digestion of adipose tissues to  separate primary


Features

Tailored Serum-Free Formulation

Exclusively designed for adipose-derived mesenchymal stem cells, providing an optimized and targeted growth environment.

High-Efficiency Cell Yield

Capable of generating 4.5x10⁶ primary cells from a mere 10 mL of adipose tissue, maximizing your cell recovery rates.

Extended Passage Durability

Supports continuous subculture up to P20 without the need for frequent medium changes, enhancing experimental longevity and reducing operational burden.

Chemically Defined & Pure System

A completely serum-free and animal-component-free formulation that ensures minimal variability and delivers high consistency across batches.

GMP-Compliant Raw Materials

All raw materials used in this product adhere to stringent GMP standards, making it highly suitable for sensitive and regulated clinical research applications.


Product Design Principle 

Based on basic media such as DMEM, MEM, and M199, various amino acids, vitamins, inorganic salts, human  albumin, transferrin, insulin, and trace elements are added. It can not only isolate primary MSCs but also  culture MSCs in the later stage, maintain the normal morphology and phenotype of cells, and avoid certain  components in serum or serum substitutes that can promote stem cell differentiation. During cell passage, it  can maintain the undifferentiated state of stem cells. 


Main Components

Amino acids, vitamins, inorganic salts, human albumin, transferrin, insulin, trace elements, etc.


Advantage

Superior Batch Consistency

A precisely defined chemical composition leads to minimal batch-to-batch variation, guaranteeing reliable and highly reproducible experimental outcomes.

Cost-Efficiency and Workflow Streamlining

Supports prolonged subculture without the need for frequent medium changes, translating to significant cost savings and increased experimental efficiency.

Optimized for Clinical Translation

Manufactured with GMP-compliant raw materials and validated through biocompatibility/toxicity testing, making it a dependable choice for sensitive clinical research and therapeutic development.

Sustained Stem Cell Potency

Actively counteracts stem cell differentiation induced by various serum components, ensuring the maintenance of the stem cells' crucial undifferentiated state and their inherent multipotency across successive passages.


Data of Continuous Subculturing

Data of Continuous Subculturing

*The above data was obtained from serum free medium and adipose tissue digestive enzymes by Yocon  Biology adipose separation technique. Different adipose samples and different separation techniques may  lead to significant differences in results. 

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