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The designated freezing medium for mesenchymal stem cells supports cryopreservation at a high density of 2x10⁷mL, and the cryopreservation density is 10 folds that of the conventional freezing medium for serums.
No serum components are added, hence ensuring higher safety profile. It is ready to use, and can be stored stably at 2-8℃.
It requires no programmed cooling, and it can be directly placed at -80° C, and after 12 h, it can be directly placed in liquid nitrogen for long-term frozen storage. The cell viability and thawed rate are both greater than 90%.
The YOCON Serum-free Cell Freezing Medium (Item No.: NC1001.1) is a specialized, designated freezing solution for mesenchymal stem cells, ensuring optimal viability and recovery post-thaw. This innovative medium is formulated with a unique blend of cryoprotectant components. Some components penetrate the cells to prevent the formation of damaging intracellular ice crystals, while others bind to extracellular water molecules, effectively reducing electrolyte concentration and inhibiting cation entry into the cells. This dual-action protective mechanism significantly enhances cell resuscitation and survival rates, consistently yielding cell viability and thawed rates exceeding 90%. Being serum-free, it guarantees a higher safety profile, minimizing risks associated with animal-derived components. The product is supplied in a 100 mL bottle and maintains stability at 2-8℃ for up to 12 months, protected from light.
Specification | Detail |
Product Name | Serum-free Cell Freezing Medium |
Item No. | NC1001.1 |
Specification | 100 mL/bottle |
Storage Conditions | At 2-8℃, protected from light for 12 months |
Cell Viability | Exceeds 90% (post-thaw) |
Cryopreservation Density | Up to 2x10⁷ cells/mL |
Cooling Method | Direct -80°C, then liquid nitrogen |
Serum-Free Formulation: Ensures a higher safety profile and reduces the risk of contamination from animal-derived components.
No Programmed Cooling Required: Allows direct transfer of cells to -80°C for 12 hours, followed by long-term storage in liquid nitrogen.
High-Density Storage: Capable of cryopreserving cells at concentrations up to 2x10⁷ cells/mL, maximizing storage efficiency.
Stable and Reliable: Maintains effectiveness for 12 months when stored at 2-8℃ and protected from light.
Enhanced Safety and Purity: The serum-free formulation eliminates potential variables and contaminants often associated with animal-derived serum, making it ideal for sensitive applications requiring high purity and consistency, such as clinical research.
Streamlined Workflow: Our innovative formula and simplified freezing protocol streamline laboratory processes. You can directly transfer cells to -80°C without the need for cumbersome programmed freezers, saving both time and resources.
Superior Cell Viability and Recovery: We consistently achieve over 90% cell viability and recovery rates post-thaw. This ensures that your valuable cell samples remain robust and functional, providing reliable and reproducible results for all your downstream applications.
Cost and Space Efficiency: By allowing high-density cell cryopreservation (up to 2x10⁷ cells/mL), our medium helps you maximize storage capacity, potentially reducing the need for additional freezer space and associated costs.
Immune and Stem Cell Storage: Suitable for long-term low-temperature preservation of various immune cells and stem cells.
Cell Preservation Centers: An optimal freezing solution for the long-term preservation of various cell lines in cell preservation centers.
Scientific Research and Universities: Widely used in scientific research institutions and university laboratories for the low-temperature preservation and management of cell lines.
Hospital Sample Banks: Provides safe and efficient cell sample preservation solutions for hospital sample banks.
Intracellular Protection: Some cryoprotectants effectively penetrate the cell membrane and enter the cell interior. By lowering the freezing point and increasing the viscosity of the intracellular fluid, they inhibit the formation of intracellular ice crystals, thereby preventing physical damage to cellular structures by ice crystals.
Extracellular Protection: Other cryoprotectants do not enter the cells but preferentially bind to extracellular water molecules before ice crystal formation. This reduces the electrolyte concentration of the extracellular solution and decreases the number of cations entering the cells, thereby mitigating osmotic shock and damage caused by cell dehydration.
YOCON BIOTECH COMPANY's Serum-free Cell Freezing Medium, by combining these two types of cryoprotectants, provides dual protection both inside and outside the cells, significantly improving the survival rate and activity of cells during the freezing and thawing processes.
The YOCON Serum-free Cell Freezing Medium (Item No.: NC1001.1) is a specialized, designated freezing solution for mesenchymal stem cells, ensuring optimal viability and recovery post-thaw. This innovative medium is formulated with a unique blend of cryoprotectant components. Some components penetrate the cells to prevent the formation of damaging intracellular ice crystals, while others bind to extracellular water molecules, effectively reducing electrolyte concentration and inhibiting cation entry into the cells. This dual-action protective mechanism significantly enhances cell resuscitation and survival rates, consistently yielding cell viability and thawed rates exceeding 90%. Being serum-free, it guarantees a higher safety profile, minimizing risks associated with animal-derived components. The product is supplied in a 100 mL bottle and maintains stability at 2-8℃ for up to 12 months, protected from light.
Specification | Detail |
Product Name | Serum-free Cell Freezing Medium |
Item No. | NC1001.1 |
Specification | 100 mL/bottle |
Storage Conditions | At 2-8℃, protected from light for 12 months |
Cell Viability | Exceeds 90% (post-thaw) |
Cryopreservation Density | Up to 2x10⁷ cells/mL |
Cooling Method | Direct -80°C, then liquid nitrogen |
Serum-Free Formulation: Ensures a higher safety profile and reduces the risk of contamination from animal-derived components.
No Programmed Cooling Required: Allows direct transfer of cells to -80°C for 12 hours, followed by long-term storage in liquid nitrogen.
High-Density Storage: Capable of cryopreserving cells at concentrations up to 2x10⁷ cells/mL, maximizing storage efficiency.
Stable and Reliable: Maintains effectiveness for 12 months when stored at 2-8℃ and protected from light.
Enhanced Safety and Purity: The serum-free formulation eliminates potential variables and contaminants often associated with animal-derived serum, making it ideal for sensitive applications requiring high purity and consistency, such as clinical research.
Streamlined Workflow: Our innovative formula and simplified freezing protocol streamline laboratory processes. You can directly transfer cells to -80°C without the need for cumbersome programmed freezers, saving both time and resources.
Superior Cell Viability and Recovery: We consistently achieve over 90% cell viability and recovery rates post-thaw. This ensures that your valuable cell samples remain robust and functional, providing reliable and reproducible results for all your downstream applications.
Cost and Space Efficiency: By allowing high-density cell cryopreservation (up to 2x10⁷ cells/mL), our medium helps you maximize storage capacity, potentially reducing the need for additional freezer space and associated costs.
Immune and Stem Cell Storage: Suitable for long-term low-temperature preservation of various immune cells and stem cells.
Cell Preservation Centers: An optimal freezing solution for the long-term preservation of various cell lines in cell preservation centers.
Scientific Research and Universities: Widely used in scientific research institutions and university laboratories for the low-temperature preservation and management of cell lines.
Hospital Sample Banks: Provides safe and efficient cell sample preservation solutions for hospital sample banks.
Intracellular Protection: Some cryoprotectants effectively penetrate the cell membrane and enter the cell interior. By lowering the freezing point and increasing the viscosity of the intracellular fluid, they inhibit the formation of intracellular ice crystals, thereby preventing physical damage to cellular structures by ice crystals.
Extracellular Protection: Other cryoprotectants do not enter the cells but preferentially bind to extracellular water molecules before ice crystal formation. This reduces the electrolyte concentration of the extracellular solution and decreases the number of cations entering the cells, thereby mitigating osmotic shock and damage caused by cell dehydration.
YOCON BIOTECH COMPANY's Serum-free Cell Freezing Medium, by combining these two types of cryoprotectants, provides dual protection both inside and outside the cells, significantly improving the survival rate and activity of cells during the freezing and thawing processes.
