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The Mononuclear Cell Freezing Medium is a specialized cryopreservation solution meticulously developed to safeguard the viability and functionality of precious mononuclear cells sourced from peripheral blood and umbilical cord blood. This advanced medium provides unparalleled protection against freezing-induced damage, ensuring that your cells maintain their critical characteristics, including a high resuscitation rate and an activated state comparable to fresh samples. Available in both Chemical Defined grade (NC1014.1) and Research grade (NC1014.2), it caters to diverse laboratory needs from preclinical research to potential clinical applications. With its robust formulation, you can reliably bank your mononuclear cells, confident that they will perform optimally upon thawing for downstream applications, including immune cell expansion and therapeutic development.
Product information
Parameter | Chemical Defined Grade (NC1014.1) | Research Grade (NC1014.2) |
Product Name | Freezing Medium for Mononuclear Cells (CD Grade) | Freezing Medium for Mononuclear Cells (Research Grade) |
Catalog Number | NC1014.1 | NC1014.2 |
Specification | 100 mL/bottle | 100 mL/bottle |
Storage Conditions | 2-8℃ | 2-8℃ |
Recommended Cell Density for Freezing | 3E6 ~ 2E7 cells/mL | 3E6 ~ 2E7 cells/mL |
Expected Post-Thaw Resuscitation Rate | > 70% | > 70% |
Expected Post-Thaw Viability | > 90% | > 90% |
Optimized for Mononuclear Cells
Specifically formulated for the robust cryopreservation of peripheral blood and umbilical cord blood mononuclear cells.
High Post-Thaw Viability
Consistently achieves a cell resuscitation rate of over 70% and viability exceeding 90% across various cell densities.
Preserves Cell Functionality
Ensures that the activated state of cells after resuscitation remains comparable to that of fresh samples, critical for functional studies and therapeutic applications.
Dual-Grade Availability
Offered in both Chemical Defined (CD) grade for sensitive applications and Research grade for standard laboratory use, providing flexibility to meet specific experimental and regulatory needs.
Reliable Cell Banking
Provides excellent protection during the freezing and thawing processes, ensuring your valuable mononuclear cell samples remain viable and functional for long-term storage and future research or clinical use.
Consistent Experimental Outcomes
By maintaining high cell viability and preserving their functional integrity, the medium helps achieve more consistent and reliable results in downstream assays, cell expansion, and immunotherapy applications.
Enhanced Research Efficiency
Minimize cell loss during cryopreservation means fewer cells are needed initially, leading to more efficient use of resources and accelerating your research timelines.
Quality Assurance
Backed by performance data demonstrating high recovery rates and maintained cell activation, offering confidence in the quality and reliability of your cryopreserved samples.
Performance Display
*When mononuclear cells are cryopreserved at three different density gradients, the cell recovery rate is above 70% and the cell viability is above 90% for all gradients.
*After cryopreserving mononuclear cells at three different density gradients, the induced NK cells, upon recovery, exhibited a positivity rate above 85% on Day 7 of culturing and above 90% on Day 14. The cell activation efficiency was consistent with that of fresh samples.
Culturing Examples of Cryopreserved Samples
*Cryopreserved peripheral blood mononuclear cell samples were cultured in a 2-liter system. After 14 days of culturing, a total of 6.48 billion cells were harvested, with a CD3- CD56+ ratio reaching 96.27%.
*Cryopreserved umbilical cord blood mononuclear cell samples were cultured in a 3-liter system. After 18 days of culturing, a total of 9.81 billion cells were harvested, with a CD3- CD56+ ratio reaching 81.24%.
The Mononuclear Cell Freezing Medium is a specialized cryopreservation solution meticulously developed to safeguard the viability and functionality of precious mononuclear cells sourced from peripheral blood and umbilical cord blood. This advanced medium provides unparalleled protection against freezing-induced damage, ensuring that your cells maintain their critical characteristics, including a high resuscitation rate and an activated state comparable to fresh samples. Available in both Chemical Defined grade (NC1014.1) and Research grade (NC1014.2), it caters to diverse laboratory needs from preclinical research to potential clinical applications. With its robust formulation, you can reliably bank your mononuclear cells, confident that they will perform optimally upon thawing for downstream applications, including immune cell expansion and therapeutic development.
Product information
Parameter | Chemical Defined Grade (NC1014.1) | Research Grade (NC1014.2) |
Product Name | Freezing Medium for Mononuclear Cells (CD Grade) | Freezing Medium for Mononuclear Cells (Research Grade) |
Catalog Number | NC1014.1 | NC1014.2 |
Specification | 100 mL/bottle | 100 mL/bottle |
Storage Conditions | 2-8℃ | 2-8℃ |
Recommended Cell Density for Freezing | 3E6 ~ 2E7 cells/mL | 3E6 ~ 2E7 cells/mL |
Expected Post-Thaw Resuscitation Rate | > 70% | > 70% |
Expected Post-Thaw Viability | > 90% | > 90% |
Optimized for Mononuclear Cells
Specifically formulated for the robust cryopreservation of peripheral blood and umbilical cord blood mononuclear cells.
High Post-Thaw Viability
Consistently achieves a cell resuscitation rate of over 70% and viability exceeding 90% across various cell densities.
Preserves Cell Functionality
Ensures that the activated state of cells after resuscitation remains comparable to that of fresh samples, critical for functional studies and therapeutic applications.
Dual-Grade Availability
Offered in both Chemical Defined (CD) grade for sensitive applications and Research grade for standard laboratory use, providing flexibility to meet specific experimental and regulatory needs.
Reliable Cell Banking
Provides excellent protection during the freezing and thawing processes, ensuring your valuable mononuclear cell samples remain viable and functional for long-term storage and future research or clinical use.
Consistent Experimental Outcomes
By maintaining high cell viability and preserving their functional integrity, the medium helps achieve more consistent and reliable results in downstream assays, cell expansion, and immunotherapy applications.
Enhanced Research Efficiency
Minimize cell loss during cryopreservation means fewer cells are needed initially, leading to more efficient use of resources and accelerating your research timelines.
Quality Assurance
Backed by performance data demonstrating high recovery rates and maintained cell activation, offering confidence in the quality and reliability of your cryopreserved samples.
Performance Display
*When mononuclear cells are cryopreserved at three different density gradients, the cell recovery rate is above 70% and the cell viability is above 90% for all gradients.
*After cryopreserving mononuclear cells at three different density gradients, the induced NK cells, upon recovery, exhibited a positivity rate above 85% on Day 7 of culturing and above 90% on Day 14. The cell activation efficiency was consistent with that of fresh samples.
Culturing Examples of Cryopreserved Samples
*Cryopreserved peripheral blood mononuclear cell samples were cultured in a 2-liter system. After 14 days of culturing, a total of 6.48 billion cells were harvested, with a CD3- CD56+ ratio reaching 96.27%.
*Cryopreserved umbilical cord blood mononuclear cell samples were cultured in a 3-liter system. After 18 days of culturing, a total of 9.81 billion cells were harvested, with a CD3- CD56+ ratio reaching 81.24%.
