Serum-Free T Cell Expansion Medium
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Serum-Free T Cell Expansion Medium

  •  The basic medium uses a recombinant human albumin system, with completely defined chemical composition. 

 
  • It supports the culture protocol without the addition of autologous plasma or serum. Without the addition of autologous plasma, when cultured for 14 to 16 days, the total cell expansion is higher than 1000 times, and the proportion of CD3+ is above 99%. In the case of adding autologous plasma, when cultured for 14 to 16 days, the total cell expansion is higher than 2000 times, and the proportion of CD3+ is above 99%.

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Product Overview

Product Name: Serum-Free T Cell Expansion Medium
Item No.: NC0105
Packaging: 1L/bottle
Storage Conditions: Store at 2-8°C for 12 months


Product Advantage

  1. Advanced Composition: The medium uses a recombinant human protein system with fully defined chemical components, ensuring consistent and reliable cell culture conditions.

  2. No Need for Autologous Plasma: The medium supports culture protocols without adding autologous plasma or serum. After culturing for 14-16 days, the total cell expansion can exceed 1000-fold, and the proportion of CD3+ cells is over 99%.

  3. Enhanced with Autologous Plasma: When autologous plasma is added, the expansion can surpass 2000-fold, maintaining a high percentage of CD3+ cells over 99%.

  4. Standardized Operation: The culture process is standardized, ensuring uniform cell states with no need for cell counting when the recommended replenishing protocol is followed.


Performance Data

  • Cell Expansion: Continuous culturing of five samples without autologous plasma results in a total cell expansion exceeding 1000 times.

  • Growth Phase: The exponential growth phase typically begins on day 7. After 14 days, with an inoculation of 3E5, a total cell number of 4E9 or more can be achieved.

    Date


Recommended Usage

For optimal results, follow the standardized culturing procedures and replenish the medium as per the recommended protocol. There is no need to perform cell counting, as the standardized process ensures uniform growth. This product is ideal for T Cell expansion and activation in various research applications, providing a reliable and efficient serum-free culture system.


The Serum-Free T Cell Expansion Medium is an advanced, serum-free solution designed for efficient expansion and activation of T cells. Using a recombinant human protein system, it eliminates the need for autologous plasma or serum, ensuring high-quality, consistent results. With proven cell expansion of over 1000-fold in just 14-16 days, and a 99% CD3+ cell proportion, this medium is ideal for researchers requiring standardized, reproducible T cell cultures. Additionally, when autologous plasma is added, expansion can exceed 2000-fold. This medium offers a streamlined, reliable, and cost-effective approach to T cell research and clinical applications, ensuring optimal cell growth without the need for manual cell counting.


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