Stem Cell Cryopreservation Enzyme Solution
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Stem Cell Cryopreservation Enzyme Solution

  • Artificial recombinant system, without human or animal derived components, and the endotoxin in the products  can be controlled at <0.25EU/mL. It has been filed with the FDA DMF, with the filing No. of 37790, which greatly  reduces the workload and difficulty in submitting the application for stem cell drugs. 

 
  • The conventional digestion time is 2 min, and the limit digestion time is up to 15 min, which has solved the  problem that the digestion time and digestion strength are difficult to control when the cells are digested by  traditional trypsin. After digestion, there is no need to stop. 

 
  • The product can be obtained by diluting the supernatant of cell culture,  which can greatly reduce the use cost.

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Stem Cell Cryopreservation Enzyme Solution is a specialized enzyme solution designed to optimize the preservation and cryopreservation of stem cells and immune cells. This product is crucial for cell preservation centers, scientific research institutions, and hospital sample banks, offering dual protection for cells during freezing and thawing processes. It combines multiple cryoprotectants to prevent ice crystal formation and minimize cell damage, significantly improving post-thaw recovery rates. Free from animal-derived components, this enzyme solution ensures high safety standards and is FDA DMF filed, making it an ideal choice for long-term stem cell storage and research applications.


Main Components

The Stem Cell Cryopreservation Enzyme Solution is designed with gentle digestive enzymes that ensure high cell viability, maintaining above 95% even under extreme digestion conditions. This solution significantly enhances yield, producing more viable cells post-digestion compared to conventional products. Its convenience is unmatched, as it is ready-to-use, requires no termination after digestion, and can be stored at -20°C. Additionally, it offers cost-effective benefits by eliminating the need for digestion termination and virus testing. Most importantly, this solution is completely free from animal-derived components, ensuring safety and clarity in ingredients. Overall, it provides superior performanceconveniencecost-effectiveness, and safety for stem cell cryopreservation.

Main Components

* The above data was the corresponding data obtained from P4 cells obtained by inoculating P2 seed cells  of MSC of umbilical cord origin in T25 culture flasks with an inoculation density of 8,000 cells/cm² and  subculturing the P3 cells obtained by digestion after 72 h.


Product Advantage

High Cell Viability

The Stem Cell Cryopreservation Enzyme Solution ensures high cell viability, maintaining over 95% viability even under extreme digestion conditions, resulting in superior cell recovery after thawing.

Enhanced Yield

This solution significantly improves cell yield, producing more viable cells post-digestion compared to conventional products, optimizing the overall efficiency of cell storage.

Ready-to-Use Convenience

The solution is ready-to-use, requiring no termination after digestion, which streamlines the process and saves time in cell preservation workflows.

Cost-Effective

By eliminating the need for digestion termination and virus testing, this product offers a cost-effective solution for long-term stem cell storage and immune cell preservation.

Animal-Derived Component-Free

Completely free from animal-derived components, the product ensures safety, clarity in ingredients, and minimizes the risk of contamination or immune responses.

Storage Flexibility

The enzyme solution can be easily stored at -20°C, providing convenience and flexibility for long-term storage without compromising product performance.

Stem Cell Cryopreservation Enzyme Solution

Product Application

Stem Cell Storage

The Stem Cell Cryopreservation Enzyme Solution is essential for the long-term preservation of stem cells. It ensures high cell viability and recovery post-thawing, making it a valuable solution for stem cell banks and research institutions.

Immune Cell Preservation

This enzyme solution is ideal for the storage of immune cells, providing dual protection during cryopreservation. It effectively prevents ice crystal formation and minimizes cell damage, ensuring immune cell survival even after extended storage periods.

Scientific Research and Development

The solution is widely used in scientific research institutions and universities for cryopreserving cell lines. It enhances the reproducibility and consistency of experiments, ensuring that research samples remain viable for long-term studies.

Hospital Sample Banks

Hospitals can utilize this product for preserving biological samples in their sample banks. It ensures safe and efficient preservation of various cell types, supporting diagnostic and therapeutic applications with improved post-thaw outcomes.

Cost-Effective and Safe Solution

The solution eliminates the need for digestion termination and virus testing, offering a cost-effective and safe alternative for stem cell and immune cell preservation. It is free from animal-derived components, ensuring safety and clarity in ingredient composition.


Working Principle

  • Cryoprotectant Components: The Stem Cell Cryopreservation Enzyme Solution contains multiple cryoprotectants that work together to protect cells during freezing and thawing.

  • Prevention of Ice Crystal Formation: Some cryoprotectants penetrate the cells, preventing ice crystal formation inside the cells, thereby minimizing cell damage caused by ice formation.

  • External Protection: Other cryoprotectants bind to water molecules outside the cells before ice crystals form, reducing electrolyte concentration in the extracellular solution and decreasing the influx of cations into the cells.

  • Dual Protection: The solution offers dual protection—inside and outside the cells—providing superior protection and enhancing cell recovery after thawing.

  • Serum-Free Formula: The product is serum-free, ensuring a cleaner, safer solution for stem cell and immune cell preservation without animal-derived components.

  • Increased Cell Viability: This combination of cryoprotectants significantly improves cell viability and post-thaw recovery, ensuring better results for stem cell cryopreservation.


Storage and Handling

  • Storage Temperature: Store the Stem Cell Cryopreservation Enzyme Solution at 2-8°C for optimal performance.

  • Avoid Light Exposure: Keep the solution in a light-protected environment to maintain its effectiveness and prevent degradation.

  • Shelf Life: The product is stable for up to 12 months when stored under recommended conditions.

  • Freezing/Thawing: Can be stored at -20°C for extended periods without affecting performance.

  • Handling Precautions: Ensure the solution is used in a sterile environment to prevent contamination.


The Stem Cell Cryopreservation Enzyme Solution is a highly efficient, ready-to-use enzyme solution designed for the long-term preservation of stem cells and immune cells. It ensures superior cell viability and post-thaw recovery with a unique combination of cryoprotectants, offering dual protection during freezing and thawing. Free from animal-derived components, this serum-free solution eliminates the need for digestion termination and virus testing, making it a cost-effective and safe option for stem cell banks, scientific research, and hospital sample banks. Optimize your cell preservation process with a solution that guarantees superior performance and increased yield.


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