Automatic Immune Cell Culture Workstation
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Automatic Immune Cell Culture Workstation

  • The Yocon automatic immune cell culture workstation is a fully automatic immune cell culture equipment developed based on Yocon's high-performance version of NK bagged serum free culture medium and the automated piping system made of FEP material. 

 
  • The overall equipment is highly intelligent. Once the cells are inoculated, no manual intervention is required, and the immune cells will be automatically harvested after 14 to 16 days of culturing. 

 
  • It has the built-in functions of real-time cell density monitoring and data analysis, which can monitor cell growth and evaluate the culturing quality in real time, and can also produce high quality results for samples at different levels.

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Product information

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1、Reagent Storage Area 

*Store the high-performance version of NK cell bagged culture medium stably at 2 - 8°C to prevent the performance degradation caused by repeated pre-warming of the reagents during artificial culturing.

2、Human-Computer Interaction Area 

*The operation interface is simple and easy to use, allowing for real-time viewing and management of data records.

3、Cell Culture Area

 *The cell culture environment is set at 37°C with 5% CO₂ to achieve the automated cultivation of immune cells.  A built-in non-contact density monitoring module monitors the cell density in real time, ensuring stable  culturing results can also be achieved for samples in different states.

Why do immune cells require automated culturing?

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Why wasn't automated immune cell culture popular before?

1、Reagent Reasons 

*Compared with the culturing methods of other types of cells, the culturing of immune cells is relatively  complicated and requires higher experience from the operators. There has been a lack of a high-quality  culture medium that can be used for programmed culturing.

2、Consumable Reasons 

*In immune cell culturing, it is necessary to determine whether to introduce fresh culture medium based  on cell density. The existing cell culture consumables are made of non-transparent materials, making it  difficult to implement a non-contact automated cell density detection method.

3、Cost Reasons 

*Establishing and maintaining an automated system requires high development investment from  manufacturers to break through the dual technical barriers of reagents and consumables.

Culture Cases

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Note: Cases 1 - 5 are automated cultures of peripheral blood NK samples; Cases 6 and 7 are automated cultures  of umbilical cord blood NK samples. The last three are customer cases, all of which are automated cultures of  peripheral blood NK samples. Among them, customer cases two and three did not provide flow cytometry test  diagrams.

Core Technology

1、High-Performance Version of NK Cell Bagged Culture Medium

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*Cell expansion of over 200 times and a positive rate of over 85%  can be achieved after 14 to 16 days of culturing. It is not selective  about samples. Both peripheral blood and umbilical cord blood can  be cultured, and both fresh samples and frozen samples are also  culturable. 

*Induced by pure factors without trophoblast cells, the culturing  method is simple and easy to carry out. The cell states are basically  uniform. Stable results can be achieved by replenishing the medium  according to the recommended procedures. 

*It can be used in combination with the research-grade basal  medium (NC0102) or the chemically defined basal medium (NC0102. H). The basal medium has been filed with the US FDA DMF. (Filing  Number: 37874)

2、Cell Culture Bags and Piping Systems Made of FEP Material

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*FEP is the abbreviation of Fluorinated Ethylene Propylene  Copolymer. The cell culture bags developed with this material have  stronger oxygen permeability and water vapor barrier properties  compared to EVA, and a higher cell count can be obtained under  the same culture volume. 

*The light transmittance of FEP material is over 95%, allowing for a  very intuitive observation of the cells inside the bags, achieving a  technological breakthrough in non-contact cell density monitoring. 

*FEP material is a 100% inert materia. No additives and other  biological source materials are added during the processing, so it  has an excellent performance in biocompatibility. Meanwhile, it can  withstand a wide range of working temperatures (-196°C to 137°C)  and meet the requirements of USP Class VI. FEP cell culture bags adopt a complex laser welding process, which  solves the possible liquid leakage problem of cell culture bags and  ensures the integrity of the sealing to the greatest extent.

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The pipeline systems that come with the equipment are all disposable presterilized pipeline systems. The materials involved are FEP, PP, PC, and TPE, all of  which are of medical - grade and have passed biocompatibility verification.

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3、Non - contact Cell Density Monitoring Module

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*The module can emit infrared light beams. After the cells are evenly shaken in the shaking platform, the  optical - signal - processing chip processes the refractive signals generated by the infrared light passing  through the cells, forming accurate non - contact cell - density - monitoring records, making accurate  judgments on the culturing levels of different samples, and adjusting the amount of fluid replacement.


Culture Cases

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*Note: Cases 1 - 5 are automated cultures of peripheral blood NK samples; Cases 6 and 7 are automated cultures  of umbilical cord blood NK samples. The last three are customer cases, all of which are automated cultures of  peripheral blood NK samples. Among them, customer cases two and three did not provide flow cytometry test  diagrams.


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