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The Fungi Detection Kit is a cutting-edge diagnostic tool engineered for the quick and qualitative detection of fungal contaminants. Utilizing the highly specific and sensitive quantitative Polymerase Chain Reaction (qPCR) method, this kit allows for the efficient screening of valuable biological samples such as cell culture media, cellular therapeutic products, and other biological preparations. It's designed to deliver results in as little as two hours, significantly reducing waiting times compared to traditional culture-based methods. The kit employs FAM and HEX fluorescent probes to target specific fungal sequences and an internal control respectively, ensuring high specificity and the reliable identification of fungal presence while eliminating false-negative results. Its comprehensive detection capability covers a wide spectrum of fungal species, including common contaminants like Candida albicans, Aspergillus niger, Candida tropicalis, Candida glabrata, Penicillium, and Trichoderma, without cross-reactivity with host cells.
Component | Details |
Product Name | qPCR Fungi Detection Kit (50 Tests) |
Catalog Number | FU0001-50 |
Tests Per Kit | 50 Tests |
Method | qPCR |
Kit Components | 5×Fu Reaction Solution, Enzyme, Fu Positive Control, Fu Internal Control, PCR Dilution Buffer |
Shipping & Storage Conditions | -20℃ |
Shelf Life | 12 months |
Detection Range | Approximately 500 types of fungi, including common species |
Reaction Time | ≤ 2 hours |
Origin | China |
OEM | Yes |
Sample Shipping | Yes |
Delivery Time | At least 30-45 days (depends on order quantity and season) |
Shipping Methods | Multiple options available |
Rapid Results
Delivers qualitative detection of fungal contamination in just 2 hours, enabling quick decision-making and reducing turnaround time for critical samples.
Broad-Spectrum Fungal Detection
Capable of detecting approximately 500 types of fungi, including clinically relevant species like Candida albicans, Aspergillus niger, Candida tropicalis, Candida glabrata, Penicillium, and Trichoderma, ensuring comprehensive coverage.
High Sensitivity
Verified detection limit of 50 CFU/mL for common fungal contaminants such as Candida albicans and Aspergillus niger, ensuring even low levels of contamination are identified.
Specific Fluorescent Probes
Utilizes FAM and HEX fluorescent probes to specifically target fungal sequences and an internal control, providing clear and unambiguous detection signals.
Internal Control Assurance
Includes an internal control to monitor the entire PCR process, effectively ruling out false-negative results due to inhibition or reaction failure.
No Cross-Reactivity
Engineered to show no cross-reaction with common mammalian cells (e.g., stem cells, NK cells, 293 cells), ensuring that results are specific to fungal contamination and not influenced by host DNA.
Enhanced Reliability
The integrated internal control and high specificity of the probes guarantee highly reliable results, minimizing the risk of both false positives and false negatives, which is crucial for product safety and regulatory compliance.
Improved Efficiency
Its rapid 2-hour turnaround time significantly accelerates quality control processes, allowing for quicker release of materials or immediate corrective action, thereby improving overall workflow efficiency.
Ease of Use
Designed for straightforward implementation in various laboratory settings, the kit simplifies the detection process, making it accessible for both experienced molecular biologists and general lab technicians.
Broad Applicability
With its ability to detect a wide range of fungal species across various sample types, this kit offers a versatile solution for diverse quality control needs in biotechnology and pharmaceutical industries.
Cell Culture Quality Control
Indispensable for routine screening of cell culture media, serum, reagents, and established cell lines to prevent and identify fungal contamination that can compromise experimental integrity and research outcomes.
Biological Product Safety Testing
Crucial for the quality assurance of biopharmaceutical products, vaccines, gene therapies, and cellular therapies before clinical use or commercial distribution, ensuring product purity and patient safety.
Research and Development
Supports R&D efforts by providing a quick and reliable method to verify the sterility of new media formulations, culture systems, or experimental samples, enabling faster progression of research projects.
Performance Verification I: Specificity
*After extracting the internal control plasmid with different culture media using the Yocon magnetic bead extraction kit, the detection was carried out through the fungal qPCR system. The detection Ct values of the internal control HEX channel fluctuated between 29-31, and there was no detection in the positive FAM channel except for the positive control.
Performance Verification II: Limit of Detection and Sensitivity
*Candida albicans and Aspergillus niger were prepared at concentrations of 10 CFU/mL and 50 CFU/mL, respectively, in accordance with pharmacopoeia requirements. Extraction was performed using different batches of Yocon magnetic bead extraction kits, and detection was conducted using different batches of fungal qPCR systems. Both fungi at the two concentrations could be stably detected. The limit of detection for both fungi was 50 CFU/mL.
Performance Verification III: Repeatability and Stability Testing
*The fungal detection system was set up and tested at corresponding concentrations by different personnel at three different time points. The results showed stable detection of Candida albicans and Aspergillus niger at a concentration of 50 CFU/mL.
*For other related experimental validations, please refer to the "Technical White Paper" of Yocon Biotechnology's products.
The Fungi Detection Kit is a cutting-edge diagnostic tool engineered for the quick and qualitative detection of fungal contaminants. Utilizing the highly specific and sensitive quantitative Polymerase Chain Reaction (qPCR) method, this kit allows for the efficient screening of valuable biological samples such as cell culture media, cellular therapeutic products, and other biological preparations. It's designed to deliver results in as little as two hours, significantly reducing waiting times compared to traditional culture-based methods. The kit employs FAM and HEX fluorescent probes to target specific fungal sequences and an internal control respectively, ensuring high specificity and the reliable identification of fungal presence while eliminating false-negative results. Its comprehensive detection capability covers a wide spectrum of fungal species, including common contaminants like Candida albicans, Aspergillus niger, Candida tropicalis, Candida glabrata, Penicillium, and Trichoderma, without cross-reactivity with host cells.
Component | Details |
Product Name | qPCR Fungi Detection Kit (50 Tests) |
Catalog Number | FU0001-50 |
Tests Per Kit | 50 Tests |
Method | qPCR |
Kit Components | 5×Fu Reaction Solution, Enzyme, Fu Positive Control, Fu Internal Control, PCR Dilution Buffer |
Shipping & Storage Conditions | -20℃ |
Shelf Life | 12 months |
Detection Range | Approximately 500 types of fungi, including common species |
Reaction Time | ≤ 2 hours |
Origin | China |
OEM | Yes |
Sample Shipping | Yes |
Delivery Time | At least 30-45 days (depends on order quantity and season) |
Shipping Methods | Multiple options available |
Rapid Results
Delivers qualitative detection of fungal contamination in just 2 hours, enabling quick decision-making and reducing turnaround time for critical samples.
Broad-Spectrum Fungal Detection
Capable of detecting approximately 500 types of fungi, including clinically relevant species like Candida albicans, Aspergillus niger, Candida tropicalis, Candida glabrata, Penicillium, and Trichoderma, ensuring comprehensive coverage.
High Sensitivity
Verified detection limit of 50 CFU/mL for common fungal contaminants such as Candida albicans and Aspergillus niger, ensuring even low levels of contamination are identified.
Specific Fluorescent Probes
Utilizes FAM and HEX fluorescent probes to specifically target fungal sequences and an internal control, providing clear and unambiguous detection signals.
Internal Control Assurance
Includes an internal control to monitor the entire PCR process, effectively ruling out false-negative results due to inhibition or reaction failure.
No Cross-Reactivity
Engineered to show no cross-reaction with common mammalian cells (e.g., stem cells, NK cells, 293 cells), ensuring that results are specific to fungal contamination and not influenced by host DNA.
Enhanced Reliability
The integrated internal control and high specificity of the probes guarantee highly reliable results, minimizing the risk of both false positives and false negatives, which is crucial for product safety and regulatory compliance.
Improved Efficiency
Its rapid 2-hour turnaround time significantly accelerates quality control processes, allowing for quicker release of materials or immediate corrective action, thereby improving overall workflow efficiency.
Ease of Use
Designed for straightforward implementation in various laboratory settings, the kit simplifies the detection process, making it accessible for both experienced molecular biologists and general lab technicians.
Broad Applicability
With its ability to detect a wide range of fungal species across various sample types, this kit offers a versatile solution for diverse quality control needs in biotechnology and pharmaceutical industries.
Cell Culture Quality Control
Indispensable for routine screening of cell culture media, serum, reagents, and established cell lines to prevent and identify fungal contamination that can compromise experimental integrity and research outcomes.
Biological Product Safety Testing
Crucial for the quality assurance of biopharmaceutical products, vaccines, gene therapies, and cellular therapies before clinical use or commercial distribution, ensuring product purity and patient safety.
Research and Development
Supports R&D efforts by providing a quick and reliable method to verify the sterility of new media formulations, culture systems, or experimental samples, enabling faster progression of research projects.
Performance Verification I: Specificity
*After extracting the internal control plasmid with different culture media using the Yocon magnetic bead extraction kit, the detection was carried out through the fungal qPCR system. The detection Ct values of the internal control HEX channel fluctuated between 29-31, and there was no detection in the positive FAM channel except for the positive control.
Performance Verification II: Limit of Detection and Sensitivity
*Candida albicans and Aspergillus niger were prepared at concentrations of 10 CFU/mL and 50 CFU/mL, respectively, in accordance with pharmacopoeia requirements. Extraction was performed using different batches of Yocon magnetic bead extraction kits, and detection was conducted using different batches of fungal qPCR systems. Both fungi at the two concentrations could be stably detected. The limit of detection for both fungi was 50 CFU/mL.
Performance Verification III: Repeatability and Stability Testing
*The fungal detection system was set up and tested at corresponding concentrations by different personnel at three different time points. The results showed stable detection of Candida albicans and Aspergillus niger at a concentration of 50 CFU/mL.
*For other related experimental validations, please refer to the "Technical White Paper" of Yocon Biotechnology's products.
