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The YOCON γδT Cell Induction and Expansion Kit Set offers efficient in vitro γδT cell induction and expansion from peripheral blood. This kit uses a feeder-free, pure factor induction method for simplified workflow. Expect high performance with over 200x cell expansion and >85% positive rate in 14-16 days. Components include YC00T (200μL vial, -20℃) and YC005 (2 lyophilized vials). For continuous expansion, pair it with NK5.0 basal medium. YOCON offers OEM services, provides samples, and ships globally from China via multiple logistics options.
| Product Name | Cat. No. | Product Use | Product Specification | Storage Temperature |
| NK Cell Serum-Free Medium | NC0102 | For in vitro culture of γδT cells | 2 bottles, 1L/ bottle | 2~8℃ |
| γδT Cell Induction and Expansion Kit Set | AN0110 | YC00T: Added during initial culture | 1 vial， 200μL/ vial | -20℃ |
| YC005: Added to the culture medium | 2 vials, lyophilized powder |
Performance advantages
High Expansion Rate & Purity: Capable of expanding cells more than 200 times, achieving over an 85% positive rate within 14-16 days of culture.
Feeder-Free Induction: Utilizes a pure factor induction method, eliminating the need for feeder layer cells, which simplifies the process.
Enhanced Continuous Expansion: Demonstrates enhanced continuous expansion capability when used in conjunction with NK5.0 basal medium, supporting prolonged culture.
In Vitro Cell Expansion: Primarily used for the in vitro induction, activation, and expansion of γδT cells from peripheral blood or freshly isolated/frozen PBMCs (Peripheral Blood Mononuclear Cells).
Cellular Therapy Research: A crucial tool for laboratory research and development in the field of cellular therapy.
Downstream Assays: The expanded γδT cells can be utilized for various downstream applications.
Data and images
Sample 1: A fresh peripheral blood mononuclear cell sample from a 25-year-old healthy male. Inoculated with a total cell number of 4×10⁷ cells, after 14 days of culture, 9 billion cells were harvested, and the positive rate was 90.01%.
Sample 2: A fresh peripheral blood mononuclear cell sample from a 38-year-old healthy male. Inoculated with a total cell number of 4×10⁷ cells, after 14 days of culture, 8.1 billion cells were harvested, and the positive rate was 92.66%.
Sample 3: A cryopreserved peripheral blood mononuclear cell sample from a 35-year-old healthy male. Inoculated with a total cell number of 4×10⁷ cells, after 14 days of culture, 8.2 billion cells were harvested, and the positive rate was 90.29%.
The YOCON γδT Cell Induction and Expansion Kit Set offers efficient in vitro γδT cell induction and expansion from peripheral blood. This kit uses a feeder-free, pure factor induction method for simplified workflow. Expect high performance with over 200x cell expansion and >85% positive rate in 14-16 days. Components include YC00T (200μL vial, -20℃) and YC005 (2 lyophilized vials). For continuous expansion, pair it with NK5.0 basal medium. YOCON offers OEM services, provides samples, and ships globally from China via multiple logistics options.
| Product Name | Cat. No. | Product Use | Product Specification | Storage Temperature |
| NK Cell Serum-Free Medium | NC0102 | For in vitro culture of γδT cells | 2 bottles, 1L/ bottle | 2~8℃ |
| γδT Cell Induction and Expansion Kit Set | AN0110 | YC00T: Added during initial culture | 1 vial， 200μL/ vial | -20℃ |
| YC005: Added to the culture medium | 2 vials, lyophilized powder |
Performance advantages
High Expansion Rate & Purity: Capable of expanding cells more than 200 times, achieving over an 85% positive rate within 14-16 days of culture.
Feeder-Free Induction: Utilizes a pure factor induction method, eliminating the need for feeder layer cells, which simplifies the process.
Enhanced Continuous Expansion: Demonstrates enhanced continuous expansion capability when used in conjunction with NK5.0 basal medium, supporting prolonged culture.
In Vitro Cell Expansion: Primarily used for the in vitro induction, activation, and expansion of γδT cells from peripheral blood or freshly isolated/frozen PBMCs (Peripheral Blood Mononuclear Cells).
Cellular Therapy Research: A crucial tool for laboratory research and development in the field of cellular therapy.
Downstream Assays: The expanded γδT cells can be utilized for various downstream applications.
Data and images
Sample 1: A fresh peripheral blood mononuclear cell sample from a 25-year-old healthy male. Inoculated with a total cell number of 4×10⁷ cells, after 14 days of culture, 9 billion cells were harvested, and the positive rate was 90.01%.
Sample 2: A fresh peripheral blood mononuclear cell sample from a 38-year-old healthy male. Inoculated with a total cell number of 4×10⁷ cells, after 14 days of culture, 8.1 billion cells were harvested, and the positive rate was 92.66%.
Sample 3: A cryopreserved peripheral blood mononuclear cell sample from a 35-year-old healthy male. Inoculated with a total cell number of 4×10⁷ cells, after 14 days of culture, 8.2 billion cells were harvested, and the positive rate was 90.29%.
