γδT Cell Induction and Expansion Kit: High Expansion, High Purity, Boosting Cancer Clinical Research!

Research and Development Background

In recent years, with the in - depth exploration of immunocytotherapy, γδT cells have gradually come to the fore. As a non - traditional T - cell subset, γδT cells recognize antigens through a major histocompatibility complex (MHC) - independent pathway, thus having unique advantages in cancer immunotherapy. A large number of studies have shown that γδT cells have a strong killing ability against autologous, allogeneic, or xenogeneic tumor cells, and their concentration is often closely related to the clinical prognosis of patients.

However, the existing γδT cell culture methods can hardly meet the clinical requirements for high - purity and high - quantity effector cells. The methods for in - vitro expansion of γδT cells can be mainly divided into two approaches: those based on the K562 feeder cell line and those based on bisphosphonates. Among them, the expansion method based on the feeder cell line requires the introduction of tumor cells, which has potential safety risks and strict quality control requirements, causing certain concerns in clinical applications. The expansion method based on bisphosphonates often fails to achieve a high expansion multiple and will affect cell viability in the later stage.

Based on this, Yocon Biotech, relying on years of successful experience in the field of immune cells, has developed a γδT cell induction and expansion kit. Paired with the newly upgraded NK5.0 basal medium, it significantly enhances the continuous expansion ability of cells, with high purity and high positive rate, meeting the needs of clinical research.

Product Introduction

γδT Cell Induction and Expansion Kit

● It is used to induce and expand human fresh and cryopreserved peripheral blood mononuclear cells (PBMC) into γδT cells in vitro. After 14 - 16 days of culture, PBMC can achieve a cell expansion of more than 200 - fold with a positive rate of over 85%.
● Induced by pure factors without feeder layer cells. The culture method is simple and easy to operate, and the cell state is basically uniform. Replenish the medium according to the recommended procedure without the need for cell counting.
● Paired with the newly upgraded NK5.0 basal medium, it significantly enhances the continuous expansion ability of cells.

Product Performance

◆Cell State

◆Case Presentation

Sample 1: Fresh peripheral blood mononuclear cell sample from a 25 - year - old healthy male. Inoculated with a total of 4×10⁷ cells, after 14 days of culture, 9 billion cells were harvested with a positive rate of 90.01%.

Sample 2: Cryopreserved peripheral blood mononuclear cell sample from a 35 - year - old healthy male. Inoculated with a total of 4×10⁷ cells, after 14 days of culture, 8.2 billion cells were harvested with a positive rate of 90.29%.

Sample 3: Cryopreserved peripheral blood mononuclear cell sample from a 27 - year - old healthy male. Inoculated with a total of 4×10⁷ cells, after 14 days of culture, 7 billion cells were harvested with a positive rate of 83.51%.

Sample 4: Fresh peripheral blood mononuclear cell sample from a 38 - year - old healthy male. Inoculated with a total of 4×10⁷ cells, after 14 days of culture, 8.1 billion cells were harvested with a positive rate of 92.66%.

Sample 5: Fresh peripheral blood mononuclear cell sample from a 27 - year - old healthy male. Inoculated with a total of 6×10⁷ cells, after 14 days of culture, 8.36 billion cells were harvested with a positive rate of 89.98%.

◆Cytotoxicity Test

Sample 1: Fresh peripheral blood sample. After 14 days of culture, the positive rate of γδT cells was 90.97%.

A tumor cytotoxicity test was carried out by co - incubating with K562 cells for 20 hours. The killing rate of K562 = (the number of inoculated K562 cells - the number of remaining K562 cells after 20 - hour incubation) / the number of inoculated K562 cells. When the effector - to - target ratio was 20:1, the killing efficiency was about 56%; when the effector - to - target ratio was 40:1, the killing efficiency was about 87%, demonstrating its good tumor - killing ability.

Cytotoxicity Test of γδT Cells Cultured from Peripheral Blood

Sample 2: Fresh cord blood sample. After 14 days of culture, the positive rate of γδT cells was 79.44%.

A tumor cytotoxicity test was carried out by co - incubating with K562 cells for 20 hours. When the effector - to - target ratio was 20:1, the killing rate was about 64%; when the effector - to - target ratio was 40:1, the killing rate was about 93%. This indicates that the γδT cells cultured from cord blood using this kit also have excellent cytotoxicity.

Cytotoxicity Test of γδT Cells Cultured from Cord Blood