Quick Nucleic Acid Extraction Kit for Bacteria, Fungi, and Mycoplasma | 40 min | Yocon Cell

• Broad Compatibility: Designed for one-step extraction compatible with bacterial, fungal, and mycoplasma detection kits, enabling triple functionality for sterility testing.

  
  

• High Sensitivity:When paired with our mycoplasma detection kit, achieves detection limits below 10 CFU/mL.Compatible with bacterial and fungal kits, maintaining sensitivity below 100 CFU/mL.

• Simplified Composition: Merges multiple lysis components into two streamlined reagents (Nucleic Acid Extraction Buffer 1 & 2), minimizing operational steps and contamination risks during extraction.

• Rapid Workflow: Three-step process completes nucleic acid extraction in under 40 minutes, ideal for high-throughput applications.

• Contamination Control: Optional extension packs eliminate interference, ensuring exclusive detection of viable microorganisms.

• Robust Sample Handling:Processes diverse samples, from simple bacterial suspensions to complex cell culture mixtures (e.g., MSC, NK, HEK293 cells).Supports up to 5×10⁷ cells/mL in cell preparations.

• Superior Extraction Efficiency:Thermal lysis method eliminates reliance on magnetic beads or column-based systems, achieving higher nucleic acid yields.Demonstrates 2–3× higher sensitivity compared to traditional magnetic bead/column methods when paired with qPCR kits.

Q: How to Avoid False Positives During Detection?

A: First, implement environmental control to prevent bacterial or nucleic acid contamination in the operation area. Ideally, ensure the extraction room is free of microbial and nucleic acid contamination, and all operation workbenches must be sterile and free of nucleic acid fragments. Second, all experimental consumables used must be sterile and free of nucleic acid fragments; if uncertain, autoclave them before use. Finally, operators must strictly follow sterile procedures throughout the process, from sampling to extraction to preparing the detection system, to avoid introducing contamination due to improper handling.

Q:White Precipitate at the Bottom of the Tube After Centrifugation—Does It Affect Extraction?

A: The presence of white precipitate generally has two possible causes:

1. Significant bacterial contamination in the sample, where a large amount of bacteria forms a visible pellet after centrifugation.

2. Residual cellular debris in cell culture mixtures or cell preparations, which remains after standard cell removal procedures and settles at the bottom during centrifugation.

   Both scenarios are normal and do not affect the extraction results.

Q:Why Does the Internal Reference Fail to Amplify After Nucleic Acid Extraction, and How to Resolve It?

A: There are two potential reasons for the internal reference failing to amplify:

1. 
   

   Excessive bacterial load: The fluorescence intensity of the target gene channel is too strong, suppressing the fluorescence reaction of the internal reference channel. This does not affect result interpretation.

2. 
   

   Complex samples with residual inhibitors: The nucleic acid solution contains high levels of inhibitory substances.

   To resolve this issue, dilute the nucleic acid solution 2–10× with high-pressure purified water before loading it into the PCR reaction.