New FEP Cell Culture Bags: An Excellent Choice for Significantly Boosting Cell Culture Yields!

When culturing suspension cells such as NK, CIK, and T cells, to achieve good final culture results, the common practice is to optimize the culture process according to the characteristics of different cells. This is indeed a very important task, but the choice of cell culture consumables should not be overlooked. Cell culture bags made of different materials have a significant impact on the final outcome of cell culture. Sometimes, making the right choice can lead to excellent results. The new - generation cell culture consumable, the FEP cell culture bag, can greatly enhance the culture results of suspension cells.

About FEP Cell Culture Bags

FEP cell culture bags

● Compared with traditional EVA materials, it has a larger gas exchange area, and more cells can be obtained within the same culture time.

● FEP is a 100% inert material, which can maintain flexibility between - 196°C (liquid nitrogen) and + 137°C (autoclaving). It is made of single - layer high - purity material, non - cytotoxic and non - histotoxic, with excellent biocompatibility.

● The light transmittance of the material is over 95%, which is convenient for better evaluating the growth state of cells and ensuring cell safety.

1.What are the characteristics of Yocon Biotech's FEP cell culture bags?

① High gas permeability and water - impermeability

The membrane material of the FEP cell culture bag with high gas permeability can promote cell proliferation, thus achieving higher cell yields. During the cell culture process, the efficiency of gas exchange directly affects the growth state and proliferative ability of cells. The FEP molecular structure contains fluorine atoms, forming highly stable carbon - fluorine bonds. This structure not only endows the material with excellent chemical inertness but also creates an ideal gas diffusion channel.

 This high gas permeability ensures that gases inside and outside the culture bag can quickly reach equilibrium, providing sufficient oxygen for cell metabolism and promptly removing carbon dioxide produced by metabolism, avoiding cell growth inhibition caused by insufficient gas exchange.

At the same time, the FEP film is completely water - impermeable. Therefore, water loss during the cell culture process is negligible, eliminating the need for humidification in the incubator and naturally blocking molds and other pollution sources. In contrast, the relatively high water vapor permeability of EVA material not only leads to evaporation loss of the culture medium but also may introduce pollution risks.

② Low adsorption

Due to its highly symmetrical and chemically inert structure, the FEP culture bag has an extremely low surface energy and exhibits excellent "anti - stick" properties, hardly reacting chemically or physically adsorbing with any substances. It will not adsorb proteins, cytokines, or other active components in the culture medium during long - term culture, ensuring the stability and consistency of the culture components.

In addition, the low adsorption of FEP can prevent cells from adhering and aggregating on the surface of the culture bag. For suspension cells, being evenly suspended in the culture medium allows them to better absorb nutrients and undergo growth and division. If cells adhere to the bag wall, it will lead to local nutrient deficiency and growth limitation.

③ High light transmittance

The light transmittance of the FEP culture bag exceeds 95%, making it more transparent than glass. In contrast, the light transmittance of traditional EVA - based cell culture bags can only reach about 80%, and there may be a problem of relatively high haze. The high light transmittance of FEP enables researchers to directly observe the cell state, distribution, and density inside the bag through a microscope without opening the culture system for sampling, greatly reducing the risk of contamination and maintaining the stability of the culture environment. In practical operation, this "non - destructive observation" ability is of irreplaceable value for determining the cell growth stage, identifying early signs of contamination, and determining the optimal harvest time.

④ Biosafety

As a material exempted from the US FDA biocompatibility test, FEP has extremely high chemical inertness and will not react with any components in the culture system. The FEP culture bag is non - cytotoxic and non - histotoxic, and it will not change the components of the culture or the culture medium. It neither combines with any elements nor releases any components of the material itself into the solution. This high level of biosafety is particularly important for sensitive cell therapy products, avoiding the impact that exogenous substances introduced by the culture container may have on cell characteristics or patient safety.

2.Are FEP - based cell culture bags safe?

FEP is a 100% inert material with high chemical stability. It will not release any components of the material itself into the solution in the cell culture bag, will not change the components of the culture or the culture medium, and is non - cytotoxic and non - histotoxic, ensuring the safety and stability of the cell culture environment.

Yocon's FEP cell culture bags are made of single - layer high - purity materials, and no plasticizers or other biobased materials are added during the processing. Therefore, they perform extremely well in terms of biocompatibility and meet the requirements of USP Class VI (the highest level of biocompatibility testing in the United States Pharmacopeia).

The product has currently passed a number of biocompatibility tests, including hemolysis tests, pyrogen tests, in - vitro cytotoxicity tests, acute systemic toxicity tests, intradermal irritation tests, and skin sensitization tests.

3.What are the culture cases of FEP cell culture bags?

The following are all culture cases of FEP cell culture bags combined with Yocon's NK Cell Serum - free Culture Kit Version 5.0 reagents:

① Manual culture